FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1979_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1979_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519049
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG6480.12484370454427232No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC6430.12388040435488751No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC5930.11424740246103933No Hit
GTAGAGGGATGACCTCATGGTCCAGGTGACACACCTGCAGGGAGGAGACA5400.10403642045356026No Hit
CCCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTA5200.100183219696021No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG450.00963305548.3377654
AGCGCTA907.47342E-540.281476
GTGATCG907.477666E-540.2775889
TAGTACT1105.2560936E-639.549084
TATACTG2400.039.2744375
GTAGAAA2201.6370905E-1136.2568171
TAAGGTA2002.237357E-1036.2533264
TAATATA1458.6814725E-735.003212
AGCGAAA2506.91216E-1131.9061
GTAATAT1601.8720657E-631.7247161
GCACCGT2755.456968E-1231.6392656
AGCACCG3000.031.4195465
TACGGGC1453.487305E-529.999867
GTTGACA3400.029.8585571
GTATTAT3450.029.4258231
GTATATA1000.006066224529.0054551
AGTTATC1000.00607193928.9998658
ACCGTGT3251.8189894E-1228.9998658
ATACTGT2853.2196112E-1027.9850226
TTATACT1306.475244E-427.887174
Produced by FastQC (version 0.11.5)