FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1979_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1979_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700481
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT450.00963794248.3333326
GCGAAAG3300.035.1515162
GCTATAT1458.693478E-735.01
ATATGCG1501.1332468E-633.8333367
CGAAAGC3850.032.012993
TATGCGC1601.8760547E-631.718758
CACGATA1050.00770690127.6190479
ACTCCGC1855.8128735E-627.4324348
AGCGAAA4300.026.9767441
GTTATAC1658.415039E-526.3636361
TATATCC2901.1892553E-825.03
GTGTAGG3001.7053026E-824.1666681
TATAGTG1550.001807950323.3870965
GTACTGA3753.074092E-1023.26
TATACTG3152.861998E-823.0158735
TATGTAC2552.705161E-622.7450983
TATATAC2903.2831485E-722.53
TATTACA3554.120011E-922.4647882
GTCCTAT4900.022.1938781
ATTAGGT2653.7772388E-621.8867933
Produced by FastQC (version 0.11.5)