FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1979_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1979_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502548
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCG450.00963644648.3332339
TATACTA1153.1599155E-431.5216752
CTAGTAC950.004725129330.526253
TAGTGTA1000.006071682628.999942
GTAGCCG2903.947207E-1027.4999439
TATAGGA1855.8086825E-627.4323772
CTTATAG1358.063109E-426.8598141
GTATAGG2207.4542004E-726.3714541
GTCTTAT2001.0616492E-525.3825241
GTATTAA3451.07320375E-1025.2248691
AGCGAAA1450.001223477225.0074141
AAACGCT1500.001493258624.166616145
GTAAGAC1851.8289278E-423.5134663
TGTACCG1902.1910909E-422.8946885
TAGGAGT3253.9799488E-822.3076464
GTATGGA2653.7640311E-621.8932821
TAGGACT2353.7045116E-521.5957014
TCTAGAC2704.4376684E-621.4814383
GCGAAAG1700.003090715721.3234862
CTATACT2053.6556076E-421.2258051
Produced by FastQC (version 0.11.5)