FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1979-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1979-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631271
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2400.051.370331
GCGAAAG2550.051.176362
CGACAGC604.9437396E-448.33323145
GTATAAG1453.8380676E-1045.0141641
ATCGAGG1209.558407E-636.2499249
CGAAAGC4100.031.82923
TAGTACC1601.8756982E-631.7186814
AGATCGA1555.4973367E-528.0644577
TATACAG3453.6379788E-1227.318785
GAGTATA3357.4578566E-1125.97009145
CTAGGAC5450.025.2751733
TCTATAC4051.8189894E-1225.0616723
GTATACG1450.001223694825.0078681
GATATAT3501.2914825E-1024.8649651
GGCTAAC1500.001490928624.1742731
TCGGTGG2151.8643548E-523.6046145
TAGTACT2151.8643548E-523.60464
TATACTA1952.6133016E-422.3076445
CTATATA1650.0025950621.9766121
GTATTAG2353.697403E-521.6025411
Produced by FastQC (version 0.11.5)