FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1979-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1979-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791408
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9560.12079736368598752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCC400.00604898254.3782733
AGCGAAA3600.046.3251571
GCGAAAG3950.044.053282
CGAAAGC4550.038.2440573
GAGCGAA1901.2914825E-1038.16261
TCTATAC7950.028.2721393
GACTATA1306.477072E-427.8880541
TGGTCGT1306.48186E-427.884531145
TATATAC3153.45608E-1127.6207083
GATATAC1907.1496215E-626.7121333
TATACTG2456.431037E-826.6342545
GTTCTAT8350.026.0511171
TATACCA9750.025.2835715
TATAGGT1450.001225787325.0015034
GTATAAA3851.6370905E-1124.483541
GTAATAC2451.9102426E-623.6748923
ATTATAC1600.002173991922.6576143
CATTACC5450.022.616043
TAGTACT2252.6494085E-522.5569134
GTACAAA2953.8676626E-722.1213721
Produced by FastQC (version 0.11.5)