FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1979-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1979-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724192
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG2800.036.2599261
AGCGAAA2950.034.4162031
CATAGCG900.00362761832.2221457
ATAGCGT1204.0570088E-430.208268
CGAAAGC3500.029.0019342
TATACTG4300.028.6627245
ATCGTGC1100.00966997126.3635738
ATTATAC2756.7593646E-926.3635733
TATTATA3057.0940587E-1026.1492842
GGGTTAC1801.5169E-424.1732861
TAGCGTG1500.001493896824.1666099
CGTATGC1550.001808034623.3870413
GTATTAT5450.021.2902341
TAGATTA2857.093653E-620.350835
ATCTATA2608.069254E-519.520532
ATAAGAC3001.1048571E-519.3332863
ACTGTAC2659.345314E-519.1508988
TTCACGA1900.00588408519.080222
AATAGTC1900.005886432719.0789037
TATATAC4303.73675E-818.5464693
Produced by FastQC (version 0.11.5)