FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1979-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1979-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653337
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC6750.10331574669733996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCG700.001054858341.4406435
AGCGAAA2450.038.492391
GCGAAAG3250.033.471292
TATCCGT900.00362214532.2316136
GCTACTA1352.1412883E-532.2217459
GTCTTAT2101.4299076E-831.0900061
GTAATAT1652.373663E-630.7759671
CGAAAGC3151.8189894E-1229.9293563
CTAATAC1753.763429E-629.0084513
TCTATCG1255.143127E-429.0084513
AGGACGT1100.00965545326.3713195
GTCTAAG3108.5128704E-1025.7411861
TAGTACC2351.3258887E-624.6880464
GGGTATA3001.6940248E-824.1811161
GTACCTA2401.5926216E-624.173716
GTTGACA5500.023.7414571
CCGACTA1851.8261092E-423.5203694
CTATCGT1550.001804758323.3939134
CCGTTGG1600.002174535522.6559149
TATAGAC1952.6082504E-422.3141943
Produced by FastQC (version 0.11.5)