FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1978_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1978_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATATACACAGTGCTTAGTACAAGTACTTGTTGGTAGTAACTGCTGGTG20.1394700139470014No Hit
ACAGAAGCCTATCACAGTTCCCGAGCTGGGTGGCACGGGACTTAGGGAAG20.1394700139470014No Hit
GTACATTAGTAAGTATACAGTAAGGAGTTTTGGGGGGTGGTTGTGGGGAT20.1394700139470014No Hit
TTCCTGATGAGTGTTGGTGAAATGACAAGGAACCTCTGAAAATGCACTGG20.1394700139470014No Hit
GTGCTGGATTCAGGACACCCTGCCCCCCCTCCTCCATCCCCAAAGTCTCC20.1394700139470014No Hit
GTCGGTGAGCATGACCCAGGACTAACACACGGGTGGGTCCTGCGATCAGG20.1394700139470014No Hit
GTTCTACATGCTGGAGCATCACTCTGGCTGCTGGCGTAAGAATGGGCTGG20.1394700139470014No Hit
GAGTGGTGCAGTGCCCCTCACACGCTCACGTCTCTGCGGTCTCAGCCGCG20.1394700139470014No Hit
TTGTGGGCCAGGTTGAGCTGCATGAGGTTCTTGAGGCCCTGGAAGGTGTC20.1394700139470014No Hit
CCATCTTTGTAAATAGTCACTTCATTAAACTCTCCTCAAATTACTCTGTG20.1394700139470014No Hit
GTTGTAATAGCTGCTTTAAATCTCTGCCAAACCCAACTTCTGGGTCATTT20.1394700139470014No Hit
CCCTAGTTACTGACTAGAGGAAAAGTTGACTTTGTTTCAGTGCAGGACTA20.1394700139470014No Hit
GTGAAGAAGAGCAACAAGTTGGAAAGTAACTGGAAGAGGCTGGAGAGGCA20.1394700139470014No Hit
GTCTGAAAACAACTGAATTGTGGGATCTCTAAGGACCCTTCCAGATGGAC20.1394700139470014No Hit
CCTTGGGGGGCATCTTCCTCAGGATATTGTGGGCCAGGTTGAGCTGCATG20.1394700139470014No Hit
GAGGAAGGCTGGGTGTGGGCCTGAGTTTGGGTCTGGGCCTGGGCCTGGGT20.1394700139470014No Hit
CCCCTGTGGAGCCCTCACAGCCCTCTCCCATGGACCCGTGGTCTCTGCGG20.1394700139470014No Hit
GCGTATATGCTCGCGCCAGGAGGGCTGAGGGTGAGGATGACCCACTCTGA20.1394700139470014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTTGC100.006357185145.08
Produced by FastQC (version 0.11.5)