FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1978_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1978_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGCAGAAGTGCATCTTATGTTAATGATGCAACTTGGGGACTGCACCT20.13236267372600927No Hit
GTATTAATGCAGTAAAATGATGCAACATTTATAAATATTTACCCACCCAG20.13236267372600927No Hit
CTGAAAGGCCGCTGGGTCAGGCAGCCGGGAGCTCGGGCGGCTGGACCCAA20.13236267372600927No Hit
CAATATATCTTAAAGTACTGTACCCACTCTTCTTCTTGAGATGACATGTG20.13236267372600927No Hit
GGATAGGAATCTGAGATATTTGCTGGAATGAATGAACGGGAGATGCTGGG20.13236267372600927No Hit
GGCATGGGGCGCCCACGGCGGGAAGGCGGTGCTGGGGGTCAGGAGAGGAA20.13236267372600927No Hit
GTCTTACCCACAGACCCTAAACATAACTCTAGGTTGGTTATTCAGGCTTC20.13236267372600927No Hit
TATGTACACAGTGTCCCTGTGATATGTGTGTATATGTGGGTCGTGAGTGG20.13236267372600927No Hit
GCCTCCATGTGTGTTCCCTGGTGGGGGTGGCCTCAGAGGGGGCATGCAGA20.13236267372600927No Hit
ACTGTATCTTTAAAAATTATGCATGTGATAAAAATAACAGGAAAAATGAT20.13236267372600927No Hit
CTTCTTGCCCCTGTGGTGACGGCCGGCTCAGGAGTCAGTGGTGGCCCGGG20.13236267372600927No Hit
GTGCTGGATGGTCTGATGAGAAGAGTTCTGTGTTTAGTAGCAAGAGTGAT20.13236267372600927No Hit
CTTAAATAGCACAGTCTACATGTATAACATGAAGACCCCTCCTACCTTAA20.13236267372600927No Hit
GGCTGGGACATCACAGCAGGTGCTACAGCCTCCCGAATGCTTCCTTCTAC20.13236267372600927No Hit
GTACTGGGGCCTGCAATTCCTTGTGCTGATACATCTCTGAGGCCCGAATT20.13236267372600927No Hit
GTCTCAGCCAGATTCAAAGAAAGAGGACTCATCATCAGAAATGAGAGAGA20.13236267372600927No Hit
GGGGAGAGAAAAGAGGAGAGAGGAATGGAGTCAGCCCGTGAGGTGGGGGG20.13236267372600927No Hit
TTTCAGACCAAGGAGACCAAAAATAAGTTGACGTCATCTGTTCATAGGGG20.13236267372600927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCAG100.0064050904145.06
ACCAGCA100.0064050904145.08
Produced by FastQC (version 0.11.5)