FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1978_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1978_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGGAAGTTTCTAGAAGCAGCCAAGCCTCCTGCTTCACAGGCAGCTCG20.1943634596695821No Hit
ACCATGCTGCGATCTAGGCTAAAGCAGGCAGGAACAGCAGCCCGTAGAAC20.1943634596695821No Hit
GAAAAAAAGGAACAACTTTTTAAAAACAGCAGAACTGAGATTCTGTGAGG20.1943634596695821No Hit
CCCCAGTACAGATCAGAGCAAAGGAAAGATGAGGAATGGATCTTAAAGCA20.1943634596695821No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC20.1943634596695821No Hit
GTGCTGGCCTGGGCCACCTCCTGCTCTCAGATGGGGAAACTGAGGCTATA20.1943634596695821No Hit
CCTCTGGTCCCAAGGGGCAGCAGCTCCTTGGCCACCTGTCCAGAGGGCAC20.1943634596695821No Hit
CTTTCCATGTCCTGTAGTCACATCATACTTATTTCTTGCCATTTCCTTCT20.1943634596695821No Hit
CTTCTACCCCTGTACAAACAATTACAGAGTTATCTTCCTGAAATGCACGT20.1943634596695821No Hit
TCTGGGTACTGTGTGGGGAGTGTGGGGTGATCTTCATGCAATCCAATGAA20.1943634596695821No Hit
GTTTTGGAACATGGTAGATCATTTTCTCAACGAATGCTAAATTATTTAAG20.1943634596695821No Hit
GATGTACACTGCGGCAAACCGCTGAGCTGACCTTAGTGAGTCTAACCGGT20.1943634596695821No Hit
GTATTATCCTGCAGACATGCAGTAAAGCTCAGTAGCTGAGAAAATAACAG20.1943634596695821No Hit
GGCTCCCTCCGCCCAGGGACCACGGGGCACCCAATTCTTCCCTCATGAAT20.1943634596695821No Hit
GTCACCTTCCTCTTAGTGCTCTCCCCCACCCCACACTCCTTCTTCCCTTC20.1943634596695821No Hit
GGATCATACCTCTTCCCGTATTGTAGAATTTGGTTTGCAGATATTTGGGT20.1943634596695821No Hit
GGCAGGGCTGGTTTCTTCTGAAGCTGCTCTCTTTAGCTTGTAAATGACCA20.1943634596695821No Hit
GTAAAGCTCAGTAGCTGAGAAAATAACAGTGTCGTTAACCTCCTTAGCAC20.1943634596695821No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG20.1943634596695821No Hit
GTCCTAACACTCAGCAGAAGCTGGAAACACACATCATAAGTTTTCGAGTG20.1943634596695821No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)