FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1978-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1978-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808225
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAT100.007090685144.99957
GCGAAAG2650.051.984172
CGAAAGC2800.051.788743
AGCGAAA2800.049.205391
TGCTACG900.00362678432.2241062
ATATCGA1255.151213E-429.0016946
AATATCG1358.0786046E-426.8534225
GACCGGT1409.993128E-425.8927677
GTATATA2601.14216164E-725.1007251
TATACTG3251.4824764E-924.5398965
TATAGTA1600.002174052922.6575742
CCTAGAC4951.8189894E-1221.9709823
GCTATGC2353.7081438E-521.595678
ATCGAGG1700.003092348621.3234588
GCTTCGG2053.6656842E-421.219448
GTAGTGT4551.5279511E-1020.7154966
GTAAGAC2155.0529896E-420.2337423
GGGTAAT2908.233297E-620.0036451
TAGTATG4001.6292688E-819.9386652
GGACCGT2556.9522444E-519.9031246
Produced by FastQC (version 0.11.5)