FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1978-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1978-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789678
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3900.040.8998642
CGAAAGC4100.038.9047473
GCGAGCG1001.3912162E-436.24986145
CTTAACG1153.1568814E-431.52961
AGCGAAA5100.029.860391
CTATATA2701.647004E-724.1681022
TATACAA2252.6494135E-522.5568942
GTCTATA1650.002596617221.9751761
GGTAGTA1700.003087755321.3288481
GTATGAC2404.3593936E-521.1470873
TAGGGTG2755.2056985E-621.0921615
CGTCAGG2455.11241E-520.714205145
TATATAG2155.0528167E-420.2337595
GTATTAC3951.4068064E-820.1949081
TATATAC3601.17453965E-720.1400853
GGATTAC2908.228124E-620.0049881
CCTATAC2556.951936E-519.9031453
TATTACC5505.456968E-1219.7739012
CCTATAT1850.00504422319.5957583
GCCTATA3351.3105582E-619.4824711
Produced by FastQC (version 0.11.5)