FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1978-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1978-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907829
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCG406.655936E-572.5152055
AGCGAAA2700.053.7268071
ATAATCG657.328651E-444.624743
GCGAAAG3700.041.157282
TAATCGT750.001481786138.6747784
CGAAAGC5050.030.1548393
CCTACGC1453.4902987E-529.999689
TATACTG4350.028.3392755
ATGCTCG1658.4183404E-526.3633569
CATAGCG1100.00967162626.3633567
TTATACT3451.07320375E-1025.222684
GTATTAT5450.023.9552541
TACCGTC1902.1898399E-422.899544
GTAATAG2303.131197E-522.0747091
TTATAGG2003.098338E-421.7545623
GTGCATA2104.3012062E-420.7231981
ACACCGT3508.714596E-820.7186326
TACACTA1750.003654030920.7186325
TATAGAC3951.4080797E-820.1941073
GACCGCA1800.00430703620.1386767
Produced by FastQC (version 0.11.5)