FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1978-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1978-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653640
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG2650.043.7831732
GAGTCGT750.00148141538.6751336
TATAACG750.00148141538.6751332
AGCGAAA3850.030.1410791
CTATTCC4900.029.5983184
CGAAAGC4200.029.3516643
CAAACGC1050.007706927627.6187539
GCGGTCT1606.825066E-527.187214145
TATAGTA1907.1383147E-626.7163752
ACTCCGT1100.00966967726.3633588
ATATAGG1958.723198E-626.0353221
AAGGTCG1701.0292242E-425.593845
TATTCCA4850.025.4179365
ATAAGAC2401.5936275E-624.1719593
CCATGCA6000.024.1664129
ATATAAT3952.1827873E-1123.8696381
ATATAGT2451.9042218E-623.682281
CTATGAT4750.022.8997524
GTATACG1952.60683E-422.315991
TCCATGC6850.022.2260428
Produced by FastQC (version 0.11.5)