FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1977_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1977_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACAGAACTGGGGGCACAGTCTGAAAACAACTGAATTGTGGGATCTCTA30.28169014084507044No Hit
GAGCTTAAATAAGAAATCAAAAACTTCCAACAAGCAAAAGTCCAGGACCC20.18779342723004694No Hit
GGAGAGACCTGTCAAAGCATCTCTTGTCGGCCTGTACCCAGCAAGTAAGA20.18779342723004694No Hit
GTGCTGGTGATGTCTCCTGACTAACAAATGAAAAGACTGATCTCAAAGGA20.18779342723004694No Hit
TTCCTGGCTTGCAAGTCCAAGGCCAGGTAGTATGTAGGCCATTTCCCAGT20.18779342723004694No Hit
CCCCATGGTTTCACCTGTGAATTCTACGAATCATCCACAGAAAAAAATGA20.18779342723004694No Hit
GGGCTTTGACATAGCAAAGGACTTGGGGGACATAAAGACAGGACCGCTGA20.18779342723004694No Hit
GTCCTGTGCCTCTGGTCCCAAGGGGCAGCAGCTCCTTGGCCACCTGTCCA20.18779342723004694No Hit
GGAGCGTGTCTACAGCATTTTCCGTTCTTTCCAGGACCTCCGCAAGCCCT20.18779342723004694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTT300.001305874424.16666645-49
Produced by FastQC (version 0.11.5)