FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1977_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1977_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG40.2883922134102379No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC20.14419610670511895No Hit
GGCTCATGTCTCCTAACATCCCTCTTCTAGGAACAGGATGATTTTCTCAC20.14419610670511895No Hit
CCTGCAGGGAGGAGACACAGGAAGTTACTAGCCTGCTGGACCTCTGGAGT20.14419610670511895No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA20.14419610670511895No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT20.14419610670511895No Hit
TTACTAGCCTGCTGGACCTCTGGAGTTAGAGGCCCTGTCTTGGCACAAGA20.14419610670511895No Hit
CTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCAAGTTAGGT20.14419610670511895No Hit
GAAACAGACATCTGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGAT20.14419610670511895No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC20.14419610670511895No Hit
CAGTTAGGCAGCTGACTCTTGATTTTGGCTCAAATCAAGAACCCAAGCTC20.14419610670511895No Hit
CTCCTGGGCCTTGCTTTCCCGGGCTCCAAGTTCACATCCTGGGTTCGTCC20.14419610670511895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA400.007150518.12585-89
Produced by FastQC (version 0.11.5)