FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1977-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1977-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800579
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG4500.037.0554582
TAGTCGG1051.8534149E-434.523728
AGCGAAA4850.034.3942451
CGAAAGC6000.027.7915953
CGACCCG1050.00770764527.618977145
CTATACA1658.416662E-526.3635674
GTATATC1450.001223571125.009311
AAGCGGC1500.001494035524.1666054
TATACAT2751.9676554E-723.7272115
GGATAGT1550.001808203123.3870356
TACTGCG1902.1926766E-422.8946787
CCTATAC1902.1926766E-422.8946783
GTATAAC1600.002170073822.6646841
TATTATC2353.7080652E-521.5956882
GTTGACA5750.021.4427621
TAGTACC1700.003092308821.3234734
TATATCA1750.003658157320.7142312
CTTATAG1800.004306398320.1388382
ACACCGT2556.955406E-519.9019096
GCAACTA6200.019.878988
Produced by FastQC (version 0.11.5)