FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1977-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1977-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833661
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC12250.1469422223181845No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10900.13074858965454783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAA400.006050553654.3749589
TAATCGT553.2187035E-452.727239
GTTACGT1255.1895768E-946.405531
ACACGAC1406.6078974E-736.24997145
GTGATCG1458.69617E-734.999979
TTACGTG1801.2919736E-732.2221952
GTATATA2301.0004442E-931.5254961
GTTATGT5900.030.7239971
TAGCATG11000.027.6817952
GTATTAG2207.4756827E-726.366781
TACGAGG1658.416969E-526.3636157
GCATGTT11300.026.3052884
ATACGAG2301.102755E-625.2173716
TACGTGT2351.330005E-624.6808323
GATTATA2651.3757563E-724.622622
CTACATC4750.024.421033
AGACGGT1500.001494071924.166647145
AGGTCGG4800.024.1666478
GGTTAGA2751.9655454E-723.7300991
AATACGA2502.2784789E-623.1999825
Produced by FastQC (version 0.11.5)