FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1977-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1977-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155887
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT15200.1315007435847968No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12310.10649829957426633No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA11770.10182656263112225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTCG3150.039.126749
GTTATGT6900.034.6826971
AACGAGT850.00274224634.11743145
GTAGAAA9350.031.7953722
TACGGAT1153.1601117E-431.5256353
GTGATCG1402.7471606E-531.0712349
GGATAGC2601.10958354E-1030.6768675
CTAGACC1902.0748121E-730.5300874
TATACTG11200.030.4278665
GTTACGT2003.2478783E-729.0073451
TTACGTG2054.0357008E-728.2961792
GTAGGCT3351.8189894E-1228.1378046
GCGTGCG5200.027.8844419
GGTCGTT2350.027.7657817
GTACGGA1050.007704294327.622462
AGCGAAA5550.027.439381
AGTGTAC2259.0997673E-725.7809623
TTATGTG9350.024.81592
GTATTAT11300.024.3867071
TATTCAA8150.024.0213725
Produced by FastQC (version 0.11.5)