FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1976-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1976-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924564
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGACCCATGCCCTGCTCCCCTGAAAAGGGCCAGGGACACAGAGTGC15090.1632120653627007No Hit
ATTTTATATTGTCCCTCTGGTTCACGGATCTCTGCACTTGTTTTCTGAAG13800.1492595428764261No Hit
GGTCTGGACTATTTAATTACTTTTCCTAAGTAAAGTTGAGAGAAGAGTGG9370.10134506643131248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1650.061.5250661
GCGTACC1307.350536E-944.6153349
CGCGTAC1553.4699042E-837.4193158
AATCGGT850.002741894234.1176077
GCGCGTA1801.2922283E-732.2221877
TACCGCG900.003628110732.2221877
TATTGTC8350.032.1257137
TATATTG8800.031.3067845
TAAATCG1453.49014E-529.9999665
ATTGTCC9550.029.6072968
TTTATAT11300.027.5884653
GCGAAAG3800.026.7104972
TTTTATA12850.026.517482
GGTATAT1658.4086765E-526.3678861
CTATATG2756.7630026E-926.3636073
AGGCGTA1100.00967126726.3636077
GCTAGTA1409.994085E-425.8928282
GCTATAC2301.1014163E-625.2214551
GGCGCGT2301.1029751E-625.2173636
CAGTGCG4900.025.1530329
Produced by FastQC (version 0.11.5)