FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1976-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1976-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147231
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC5400.061.7617453
AGCGAAA5800.056.264531
GCGAAAG6400.050.978622
GTATAGG2700.040.288181
ATAGCGG1354.9706614E-737.594115
CTAACCG800.002036336836.251464
ATGCCCG1801.2927194E-732.222115145
CCTATAC5750.031.523013
TATACTG8600.030.350065
CTATACT6800.029.8541434
CATAGCG1907.154398E-626.7116034
GTGATCG1409.995686E-425.892779
CCCTATA4900.025.1540762
TAGCGGT2051.2900773E-524.7570936
ATACTGT9300.023.3880396
CGTCGGG1700.003088438921.3290371
CCGTCCG1700.003093127921.323469
GCGGTCT2404.3637134E-521.1457638
TAGTACC2104.3134924E-420.715124
GGGTAGC9200.020.4944211
Produced by FastQC (version 0.11.5)