FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1976-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1976-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087107
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA12280.1129603617675169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCA553.2184465E-452.7295275
TATAGCG3200.036.2515532
CGAAAGC4100.033.5993
ACTGATC3900.031.6024638
TATACTG7950.030.0956275
TTATACT7250.030.0012844
TCGCCAT7900.027.531559145
GCGAAAG5150.026.7487162
CGCTATA3555.456968E-1226.5504322
CTATTAG8050.026.1239381
GCGGTAT5000.026.1011186
TAGTAGA7900.025.6973065
TATTAGT9100.025.4955982
AGTAGAA9850.025.031061
GACACGT2101.5547144E-524.1677046
GTAGCCG2101.5552683E-524.1665929
CCTTATA8600.023.6056632
TATAACT2802.3420398E-723.304574
CGGTATC5600.023.3034977
ATTCGAC3803.619789E-1022.8999311
Produced by FastQC (version 0.11.5)