FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1976-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1976-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896111
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG10050.11215128482966953No Hit
CTCTAGCAGCGTGTGCCCCAGAATCACACCCCAGGGCCTCCGCTTGCTGG9930.11081216501080782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCA606.805236E-660.416349
TACGAGT1258.913048E-1152.199715145
GCGAAAG4250.051.1819042
TATAGCG858.917232E-751.1819042
CTATGCC1255.1913958E-946.404933
CGAAAGC4900.044.392473
AGCGTGT3200.043.0466468
GTCGACT1105.2638843E-639.5452428
GCGGTAT1157.136676E-637.8301056
CCGTTCA4550.036.6481559
TCGACTC1209.563095E-636.2498059
ATGGTCG1656.009941E-835.1552475
TATCTTA1458.689858E-735.0037166
TATCAGG4600.033.101342
AGCGAAA6600.031.8631
TCGCCAT5500.031.636192145
CTAATAC1652.3840876E-630.7608413
TACCGTG1652.386163E-630.757417
CCTATAC3850.030.133073
TATTAGT8650.029.3383772
Produced by FastQC (version 0.11.5)