FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1975_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1975_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGGAATGAGCTAAAAGAGAGTGGGGGTCAGGCCTCACCAGACATGG14.0No Hit
GTTAGTGAAAGATCAAATACTGCACCTTGGATAAGGTCTGTTTCCGATTG14.0No Hit
CAATATGTCTGTGCTCTTGAGAAATGGATTTGGGACAGAGAAAGTGAGAG14.0No Hit
GTGTGGGGCTCGATCCCAGCACCCTGGGATCATGACCTTAGCCAAAAGCA14.0No Hit
CCCAAGACTCTGGGATCATGACCTGAGCCGAAGGCAGCGGCCCAACCCAC14.0No Hit
GGCACAAGAGTTGGCCAGGATGGGCTCCAGGGGGGCCGTGGGAGAGACCT14.0No Hit
GACCTAGCCTGCTCTGGTGAGGGTGAGCCATCCTGGCAGAAGGCACACAT14.0No Hit
GAAAAAAGTCCTTTTACCAAAACCATCAGCTTCTTTTAGAGCACGAGGGA14.0No Hit
GAAGAAATTGAATGTAGCAGGTGGATGGCAGGCACTTCAAACCTATGGCA14.0No Hit
CTAATACTGTACAAAACCTAAAGACAAATAAACCGACATTTGACGGTGGG14.0No Hit
GGGAGAAGCATGCTCCCGATACGGGCCTCCCTCTGTCTGACTGACTGCAA14.0No Hit
CAAAAAATGTTGTAAAAAATCTTATTCTGGGAGTACCTGGGTGGCACAGT14.0No Hit
GGAGGGACCTGAACTACTGACCGTAGCATCTCAAAGGCATCATTTACGTG14.0No Hit
GTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTG14.0No Hit
GAGCCCTGGCTTTGAGGCCCCTCTTGCATCATTTAGCAGCTGTGTGTCCC14.0No Hit
CATAAACATTGACCTCACGCACAACACTATAACTTATGCTTGGATGATTT14.0No Hit
CTGGAAGTGACTGACTGTCTTAGTGTGTCAGTCGCAGAAAGGGTGCATTG14.0No Hit
CCACTGTGCTCCTGGGACTCCTAGTCTAATGGCAGAGGCGAGGTGCTGCT14.0No Hit
CCCTATGGCTCATGTCTCCTAACATCCCTCTTCTAGGAACAGGATGATTT14.0No Hit
CAGCCACACTGACCGGCCCCGTTGTGGAGGACGCGCCCACACTGGCAGCA14.0No Hit
CCTAATCCCCACTTTGTCACATAAATCCAGTTGCTGTGGAAATCATTCCG14.0No Hit
GCCTGATAGGTTGAGGCATATTTTCGAAAACTGCATGTAGGATAATGGTG14.0No Hit
AAGGTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT14.0No Hit
GGACCTATGGAGGCCTGCAAGGCAGAACCCGAGAGAGGGTTTGTGGGATG14.0No Hit
GTGAAAGGACACAAGCGGGGGAGCCGCAGAGGGAGAGGGAGAAGCAGGCT14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers