FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1975_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1975_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT15.555555555555555No Hit
GAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTT15.555555555555555No Hit
GCCCCCCTTTATCTGGATTGCCAGTTCAGGAAGGAGGGAATGGATCACAT15.555555555555555No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACGATGAAGGAATAT15.555555555555555No Hit
ACATTCTACTGCCATTCCTCTCCCTGCCCAAGGAGCCTCCTCTCCTGGCT15.555555555555555No Hit
GTCCATGGTGTATCCTGTTCCTGTTCCATGGCTATATGGGGGATCTCCAG15.555555555555555No Hit
CAGGCTGACAGATTGATCACCACCTTGGTGGTGGTTAGTCACGCAATGTT15.555555555555555No Hit
CCTGTGGCCGACTCAAGCCAGAGAGCTGTGGGCAGTGGTTAAAGCATGGC15.555555555555555No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC15.555555555555555No Hit
GATGTTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG15.555555555555555No Hit
ACCACGAACAGGCCTCGCCAGCGCAGGCTCTGAGGCATAACTGTGCGCAG15.555555555555555No Hit
GATCACGGCCTGAACTGAAACCAAGAGGCAGACGCCTAACCAACAGGGCC15.555555555555555No Hit
CTCCAAGGGAGCGGGCCTGGCCAGGCCGGGAGTGTGGCGGTTGGAGGGCG15.555555555555555No Hit
GAGGAAGGCTGGGTGTGGGCCTGAGTTTGGGTCTGGGCCTGGGCCTGGGT15.555555555555555No Hit
ACCCAACATGATCTATCACCAGCCATACATCACTGTTCCAGGACTGCACA15.555555555555555No Hit
GGTCAGAAGTGATACTCACGGTTAGGTCTTTAGAGTGCTTTTCCATGTCC15.555555555555555No Hit
ATTAGAGTCTCTCTTATGACATGAGACGTGGGGTCGGTCTCATGGCAAAG15.555555555555555No Hit
CGTAGAACCCAGGCAGTTTGTCATGAAGGACCCGCCAACGTCAAGTCCCG15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)