FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1975-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1975-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886470
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11160.1258925851974686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG950.00472701830.5263022
CGAAAGC4850.029.8968963
CCTATAC1804.6991863E-628.1944353
TATAGCG1555.4997436E-528.0645055
TGATCCG3108.58563E-1025.7257969
ATAGCGA1701.0311661E-425.5882246
GTCATAG3902.0008883E-1124.1666562
CGTATAC3001.7065759E-824.1666563
GTATTAC2802.3407301E-723.3048761
GTATAGT2603.201927E-622.308941
CCGTATA2953.8733742E-722.1186352
CATAGTG4409.822543E-1121.4204464
GTGATCG3055.33877E-721.3934369
AGCGAAA6450.021.3577861
TAGTACT1700.00309252821.323524
CATATAC7150.021.29373
GTTATAC2404.3602813E-521.1470181
TCCGTAT3508.728057E-820.7154461
AACCGCT2806.0890034E-620.7142777
GCATCGA3551.0143958E-720.422527145
Produced by FastQC (version 0.11.5)