FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1975-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1975-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074937
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGC752.549397E-548.337555
ACGCCGT951.0284137E-438.1612246
GTATAAG2109.094947E-1237.9883421
TATACTG3750.032.8695345
AGCGAAA3850.032.023231
GCGAAAG3900.031.6067922
CTAGTAC1652.3850243E-630.760263
GGTCGAA1204.0506484E-430.2179971
CCCGTAA1255.151463E-429.002533
TATACGG1255.151463E-429.002535
TACCGGG1555.4957884E-528.0682722
GGTAACG1606.828874E-527.1873428
TTAGGTA3657.2759576E-1225.8241734
GATATAC2001.0648446E-525.3772143
ATATAGG2301.1024058E-625.2195933
CGAAAGC4950.024.9011633
GTACCGC2051.2895091E-524.7582596
GTATAGG1851.8268252E-423.5210361
CCGTTCA1600.002175328622.6561189
GTAACGG2252.6518994E-522.5554269
Produced by FastQC (version 0.11.5)