FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1975-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1975-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993105
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11840.11922203593779106No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC10230.10301025571314211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1451.9244908E-840.0079351
TATACTG3000.038.668495
TTAGGTA2551.8189894E-1234.119264
GCCGTAG900.003624697732.228611
TAGTCGG1352.1422335E-532.222128
TTACGTG2051.1381417E-831.830772
TAGTACC4250.028.999919
GTTATGT6800.027.7260861
GCGAAAG3950.027.5329442
GCACGCG1100.00967170326.3635528
GTATAGG1958.740943E-626.0308021
TATAGGT2001.0650692E-525.3761984
AGCGAAA4300.025.2957121
GTACTGG3652.1827873E-1023.8403431
GTTATAG2451.911274E-623.674593
GTATTAG1550.001806395123.3917331
GGATTAG4053.274181E-1123.2762181
TATAATA7850.023.0902612
GTAATAT2202.2253704E-523.0727561
GTAACCC1600.00217454222.657323
Produced by FastQC (version 0.11.5)