FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1975-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1975-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036388
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC11990.11569026272013956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1502.5944246E-838.675681
ATAACCG750.001482065338.673815
GCTATAT2752.1282176E-1029.0067561
GCGAAAG5750.025.2232651
ATATACC2951.4251782E-824.5808094
GTATTAT11050.024.2816731
TTAGGTA3601.8371793E-1024.171134
ATTATAC3052.0309017E-823.7748833
AAGACCG2151.8631574E-523.609015
TATAATA10150.023.575782
TAACGTC1851.830832E-423.5133177
TTACGTG2802.3386201E-723.3078752
CTATATA3753.074092E-1023.2042852
TACAGCC8950.022.6813767
AGACGGT3203.3862307E-822.656061145
AGCGAAA4607.2759576E-1222.0703581
TATATAC4951.8189894E-1221.9737533
ATACAGC9700.021.6792626
TTATAAT9750.021.5691261
GTATATA3055.328602E-721.3984281
Produced by FastQC (version 0.11.5)