FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1975-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1975-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915040
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12060.13179751704843504No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT11940.13048609896835112No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC11880.12983038992830914No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9510.10392988284665151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGT450.00963900748.3331689
TATACTG3400.042.6492465
TTAGGTA2950.036.86634
GTAATAT3150.036.8333241
GTTACGT800.002034374536.25781
AGCGAAA3350.034.634321
GGTCGAA850.002741909634.117537
CCGACTA1751.00857505E-733.1445584
GTTATGT4950.032.2291561
GTATTAT7600.030.5328871
AGACGGT950.004727166630.526213145
GCTACTA950.004727166630.5262139
GCGAAAG3900.029.7451132
ATTAGGT3700.029.3933963
CTACTGA7800.027.8860446
ATCTAGA1606.817516E-527.1933521
GGTTATA1606.817516E-527.1933521
GACGACT1358.079765E-426.8532284
GATTAGG4400.026.3649862
GGATTAG4800.025.6826111
Produced by FastQC (version 0.11.5)