FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1974_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1974_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGCAGCCCGGGCCATAGGGCAGGACTGTCCCTGGAAGTGCGTGACT18.333333333333332No Hit
GTGTACATACACACCCACACACAACCACACACACACATCCAGAATGATAT18.333333333333332No Hit
CTTTCTAGGTATTGCTTCAATGCTCAGAACATTTCGACAAGGTAGAACCC18.333333333333332No Hit
GTCCCAGGATGCAAACGTGCCTCTCTGCAAACACTTCTACACTAAAACAC18.333333333333332No Hit
GGCGGAGGCGGGGGTGTCCCGGGAAACAGGGAAATTTTCCCATTGCTCCC18.333333333333332No Hit
GAGGAAGGAAAGCAATGCTCATTTTTGCAAAGGATGCCCATTCCTTATCC18.333333333333332No Hit
TTCCCAGTTGCTCCTTATTAATCTGCAGTAGCCAGTGTTGGTGGTAGCAA18.333333333333332No Hit
ATAAAGTGCTCAGCACAGAGTAAGTGCTCAGTAAAATGTAGCAATCATGG18.333333333333332No Hit
CCTCAGCAAGGCTTTGGGCGTTCGCTCTGTCGCCTCAGGGAAGAAGAGCC18.333333333333332No Hit
AGTCTGGACAGAAACTCAAACTTGATGCACACGCGGTCAGTGGTGGTCAG18.333333333333332No Hit
GATAAGAGCCTGGCTGGTTCTCCTGCCTTCGTGTCTTTGGAATTTTCCTT18.333333333333332No Hit
GACCAAATGAGGACAGGTGGACGTGACCTAAACCATTTCTGAGCAGAAGC18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)