FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1974-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1974-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200618
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12910.10752795643576893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT750.001483220138.668183
GTGATCG1553.4713594E-837.419269
GTTGACA9500.032.057891
GTTACGT1606.8216366E-527.191961
ACATGGT12350.024.6568365
TATACTG5400.024.1676125
TGACATG13500.023.6305523
CTACTGA11250.023.2009076
GGTCCTA15800.022.9429789
GGTAGCG3253.9917722E-822.3076368
GTAGCGG3055.3421354E-721.3933899
GTTATGT9500.021.3719271
GACATGG14350.021.220344
GTATACT2857.0898404E-620.3542161
TAGCATG16450.020.274352
TACTGCG1800.00430659220.1396755
GTGCGCC1800.004307630520.1388389
TACTGAA13100.019.9236137
TTACGTG2556.956877E-519.9027392
TATAATA6600.019.7734992
Produced by FastQC (version 0.11.5)