FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1974-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1974-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016737
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG19810.19483897999187597No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC15500.1524484699583078No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA12210.12009005278651214No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA11860.11664766798100197No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT11540.11350034473024981No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT10610.10435343653275135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACTA2300.044.1303759
TATACTG4700.033.9361235
GCGGACC1204.0581267E-430.208299
GTAATAT3950.029.3728261
GTATTAT10200.029.1478521
TAGTCGG1504.3988184E-528.9999628
GTTGACA10700.028.4635071
CAGGTCG6100.027.3360277
ATATATC3409.094947E-1125.5882023
GTGCTAA9250.025.0859811
ACATGGT12250.024.857115
AGGTCGG6750.024.703678
GCGAAAG3601.8371793E-1024.1713891
GCTATAT2401.5954101E-624.1713891
GTAACCC1500.001494301824.1666343
TATTATT11800.023.9618322
TAATATA4550.023.9010682
GCAGTGC8500.023.887021
CAGCTAC4600.023.6412727
AGACGGT1851.8307129E-423.513481145
Produced by FastQC (version 0.11.5)