FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1974-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1974-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962425
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT37270.3872509546198405No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA26730.2777359274748682No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA14270.14827129386705457No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13720.14255656284905316No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA13160.13673792763072448No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA13030.1353871730264696No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG11960.12426942359144869No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG11930.1239577109904668No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC11560.12011325557835675No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG11530.11980154297737487No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC9980.10369639192664364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAA11450.054.456822
CGACGAT450.00963715948.3357054
AAGGTCG4000.045.3123669
ACAAGGC13300.043.60898
GGTACTG3750.042.5354235
GGTCGTT3800.041.9735687
GTGATCG907.482359E-540.277669
AGCGAAA5100.039.8100131
GCGAAAG5300.039.6717572
TAGAAAC16300.039.5878333
GCGGACC1651.366061E-939.545349
GTCGTTT4200.037.976088
CGAAAGC5750.037.8279463
AGGTACT5300.036.9357724
GCGATTA800.002036605536.249897145
AGGTCGT4450.035.8444566
TATACTG18800.034.7091525
TTATACT17700.033.5892224
ACAGTAT6700.033.544688
CAAGGCA16900.033.4614459
Produced by FastQC (version 0.11.5)