FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1973_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1973_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA32.941176470588235No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG21.9607843137254901No Hit
GAGGCTTAACCCACTGAACCACCCAGGTGCCCTGGTTTAGCTTCTTTCTT10.9803921568627451No Hit
CCCCCAGAGCTCCCCATCCCAGTGCGCGGGCGGTGTGCAGGGATCGTGTC10.9803921568627451No Hit
GTGAGGTGGGGGGATTGCACTCTGGTAAAGATCCAGTGAATCCAGTTACA10.9803921568627451No Hit
GTTGCAAAGAGAGCGAGAATGCATCTGGTTTCCGTCGTCGTTACTGTTAT10.9803921568627451No Hit
GTAATATACTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTT10.9803921568627451No Hit
ATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGC10.9803921568627451No Hit
GCCAAGATGAGGAGAAGTGGGAGGAGCCAGTAGAGGGATGACCTCATGGT10.9803921568627451No Hit
GTCAGAGAGAGAGGGAGAGAGAGCGCGCATAGGAGGACAGAATGGCAGGC10.9803921568627451No Hit
GAGCTGAAGGCAGAGGCTTAACCCACTGAGCCACCCAGGTGCCCCCCTAG10.9803921568627451No Hit
CTCTAAGTGAAGGATAAAAGCTCTAGTGTTGTAGGGAGCAGACGGCTCCT10.9803921568627451No Hit
TAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCAT10.9803921568627451No Hit
GTGGCCCCGGGTGTGCTGTGTACCCTCTAGGGCCTGTGAGTAATGAATCT10.9803921568627451No Hit
GAGGGATCAAGTTAGGTTGCTGATTTGAGACGTTTCGTGGTTCTTGAGTT10.9803921568627451No Hit
AATCCAATGCCTGCATGTTAACAATTTTCACCTCTGAATCCTCAGTCCTG10.9803921568627451No Hit
GGGATGTCTCGATAAGGGCCGTGCTGGCCTCATAAAATGAGGTGGGGAGC10.9803921568627451No Hit
GATCCAGGGAAGCTTATTGCCCCGGCTTCCTCTGCGTGGGCCACGGGTTG10.9803921568627451No Hit
GCCTTCAATAAATATGATCAAAGGACTTAAGTTTTTTCAAGCCACAGGAC10.9803921568627451No Hit
AAGTAAAATAGGATATTTAGGTCATTAGCAAGAGTTACTGAAGAGGATTT10.9803921568627451No Hit
CCCTCCCACCACCCCCAATAAGTAAGCCATCAAGTAGATCCAGACTCAGT10.9803921568627451No Hit
GAGCTCAATCTCGTGACCTTGAGATCAAGCCCTGAACTGAAACCAAGAGG10.9803921568627451No Hit
TTTCAGGTGTTATGAGGTATTTGGGACTACAAAAATGGGTAAAATGGAAA10.9803921568627451No Hit
GAATATCACAAAGGAAACATCAGCCTCAGACAACCCGTTACTTAGATGGA10.9803921568627451No Hit
GAAGAAAGGCAGGGATTAGGGCTCTGCTCGAAGTCTTGCTGTGTGGCAAG10.9803921568627451No Hit
CAGCAGGGATGCTGCTGGAGGATACTCTCTCTCCCTCTGACCCTCCCTTC10.9803921568627451No Hit
CTTTTATATTACTCCTGTCCATTTTTTTTTTAATTTAAGGTTTAATCTTT10.9803921568627451No Hit
TTTTAGCACCTGTAATACTGCATTCTGGGAATTTTGTGTCATTTTAGTAT10.9803921568627451No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA10.9803921568627451No Hit
GCATCGAGGTCCGTCTCACACTCTGTCCTCACCATGGCGTCTGCTTGAGA10.9803921568627451No Hit
AGTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAA10.9803921568627451No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG10.9803921568627451No Hit
GTCCGACTGGCAGCGGCTGCGGCCTGGGACTGGACCCTCCCCTTCCTCGG10.9803921568627451No Hit
ATCTGTAGTGGTGCGGATCATTTCTACTTCTGGTTCTTTTCTCATCTTTG10.9803921568627451No Hit
GTGTAACAGTGTGTGAGCGATTGCGCTGGACTGATTACTCCTAGGAGCGT10.9803921568627451No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG10.9803921568627451No Hit
GAAAAGAGGAGAGAGGAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCAC10.9803921568627451No Hit
GTCTCGTACACCTCGTGCAGCTTCACGATGTTGGGGTGGGAGCGGCTCTG10.9803921568627451No Hit
CTGCTAGGGAAGAATTTCTCGAATAATGTGTTCTGTTGACTGTGTCCATG10.9803921568627451No Hit
GTGTTTTGTGTGATGGCCCTGTTATGGCACCCTTTGTTAAGATCTTCTTG10.9803921568627451No Hit
CAGCTGCTGTCAACAGAGCGCTTGGTGTGGGGGAAATCCGTTCCAAACTG10.9803921568627451No Hit
GTAGGTGTGACATTAGAAATAGAAATAGACACATAGAAATCCAAGAAATC10.9803921568627451No Hit
GAGTTAACCCCGAGGGTTGTGCTGTTGGGGCGGGGCATCCGGAGCAGTGG10.9803921568627451No Hit
GCTCATGCAAATGTCAGTAGCAACCAAAGTCTTGACCATCTAAGCCTTAT10.9803921568627451No Hit
GGAAATGACATTCCATGGAGCAAAGGAAGTTGCACTCAGTTACTCAACTG10.9803921568627451No Hit
ACTTTAGCTTGTTGGAAGTTTTTGAGTTCATATTTAAGCACTCTACTCGA10.9803921568627451No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA10.9803921568627451No Hit
CCATATAGCCATGGAACAGGAACAGGATACAGCAGTTCATATCGGAGGCC10.9803921568627451No Hit
GGCTTGAAGGGTGGATTTCAAATCATTTTTAACAACACGGCACTTCTTTC10.9803921568627451No Hit
ATTCTGTTATATTCTGTTATTCTTTGAAGAGTATTGACTTTGGTTCTACT10.9803921568627451No Hit
TACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGAT10.9803921568627451No Hit
TTCTAGAAATGTATCCTTTTCTTTTGGATTATCCAGCTTGTTGGGCTGTA10.9803921568627451No Hit
GTACCGGGCCACCTTCACCTCCCAGAGTTGCCTTCCAGTCCTGCATGCAG10.9803921568627451No Hit
TTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAAGACCCGAAA10.9803921568627451No Hit
ATCAGTTGTAGTGCCCTTCATTGGCTCTTGTTACAGTGTTTGGATTTTTT10.9803921568627451No Hit
AACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGAC10.9803921568627451No Hit
GAACAGGAACAGGATACACCATGGACACAGTCAACAGAACACATCAATAT10.9803921568627451No Hit
TTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAA10.9803921568627451No Hit
ACCCAAGCTCCTGGGAGAGAGCCCTGCACCAGGCTCCACACTCAGCGGGG10.9803921568627451No Hit
CTTTTAGACCTGCACTGGAACTAGGGTGAGTCCCAATTGGCGTCATAGCC10.9803921568627451No Hit
CTCTTCTCCCTGACCCGGCAGGGCCCCCGTTGCTCTTTTCCAGAAGACCG10.9803921568627451No Hit
CTGCCGCAGCGTGCGTGGCCTAGGATGGCACTCTGCTCACAATGCCATCT10.9803921568627451No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA10.9803921568627451No Hit
GAAACAGACATCTGACTGTTCAACTGTCTACTAATGACCATTAGAGAGAT10.9803921568627451No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG10.9803921568627451No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT10.9803921568627451No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG10.9803921568627451No Hit
CTATTTTAGTGCATGTGTGATGAAGGAGTTGAACCAAGATGCATTAAGCA10.9803921568627451No Hit
GGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATTTGAAGAACAAGAAC10.9803921568627451No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG10.9803921568627451No Hit
ATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGA10.9803921568627451No Hit
AGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGAT10.9803921568627451No Hit
GTTCTGCTCCATTGGGCTGAAAATAAGAAGATCATCTGTTAGTTCTGCAG10.9803921568627451No Hit
GGGGTTTCTGTGGTTTCTTTTCATCCCATAGTGCATTTCTGGAGAAACTG10.9803921568627451No Hit
CCAGGACCCCCCATTTCTGTAAATCACAGAATCTGGGCTTGAAGGGTGGA10.9803921568627451No Hit
GGGGTGGAGGATGGAAGGTCAAAGAAAAGGTGTTTCGAAGGATGAGAGCG10.9803921568627451No Hit
GAACAGGACACAGTCACTCGGCCCAACACTGGCCAGAGCTCCCATGAGTG10.9803921568627451No Hit
GCATATCCTACCACTGCCAATGCAGGCATCTATGCCAGATGTGGCCCCGA10.9803921568627451No Hit
TGCCTAGCCTGACTTGCGACTTCCATGGCTTCTGCTGCCTGCTCACTTGA10.9803921568627451No Hit
GAGCTGATCAGGACGTATTTAAACTCTGGACGTGTCTTATTGCAGTGAAC10.9803921568627451No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT10.9803921568627451No Hit
ACATTCATGAATTCATTAAGGAGGCCTATTAGTAACTAGGCTAGGTAGCC10.9803921568627451No Hit
GGGGGGGGGTAGACAGCACTACGGCTAAGGCAATGGAGAAGATGGCTGGA10.9803921568627451No Hit
CTCGCGAGATACTGACAAAAACCACTGTGGATCATATGGACATAATTAAG10.9803921568627451No Hit
ATTGAAGGAAGAGACCGAGCAATGGCCTGGACAGTGGTGAATAGCATCTG10.9803921568627451No Hit
GCCTTATAACAGGGAAAGGTGACGGGGAGTAGTTCAGAGGGAGGCATGGA10.9803921568627451No Hit
GGAGAGACCTGTCAAAGCATCTCTTGTCGGCCTGTACCCAGCAAGTAAGA10.9803921568627451No Hit
CTCTCTCTCCCGCTCTGTGTCAAATCAATAAATGACATCTTTAAAAAATA10.9803921568627451No Hit
GATATATATTAAAAATAAAAACACCAACAAGAATAACAGATCAACAAAGA10.9803921568627451No Hit
GCCCTAGTCAGTTCAACTCCACAGGGAGAAGAGAAGTTTCTCAAGTTATC10.9803921568627451No Hit
GGATGGGAAGCAGGCCCCCTGCTGAGCAGAGAGCGCGGTGTGGGTCTCGA10.9803921568627451No Hit
GGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTACTTAATCAATCCAG10.9803921568627451No Hit
CCCCAAAGCATTGTGTTGATAAGTTTTGTGTGACATTGGAATCTCTTTAG10.9803921568627451No Hit
GGAGAGAAAAGAGGAGAGAGGAATGGAGTCAGCCCGTGAGGTGGGGGGAT10.9803921568627451No Hit
GGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATGGACAC10.9803921568627451No Hit
CACCTAACAGACCAAGCCACTCAGGCAGCCCTTAGAGCCAACTTTAAAAA10.9803921568627451No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA10.9803921568627451No Hit
GTTAATAGAAGGAACATATCAGAACTTAAATAAATGAAATACAGACTAAA10.9803921568627451No Hit
GTGCCTAGGACAGAGTCAGGCATATAGTAAGTGCTCAATAAATATTCGCT10.9803921568627451No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers