FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1973-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1973-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688927
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACT28590.41499317053911366No Hit
GTAGTATGTAGGCCATTTCCCAGTAGTGTGACCCCACTTTCCATAGCTGG22170.32180477757440196No Hit
GATCCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCAGTAACGTT16670.24197048453609743No Hit
TTCCATAGCTGGAGAATGTATTCATCACTATTCCCATGTCATGGGGTCGG16320.2368901204336599No Hit
CTCTGGGAAAGATCCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATT14350.20829492819993992No Hit
GTCATGAAGATTCCTGGCTTGCAAGTCCAAGGCCAGGTAGTATGTAGGCC13480.19566659457388083No Hit
ACCCAGGACTAACACACGGGTGGGTCCTGCGATCAGGGCCCTGCGCATGG13360.1939247554530451No Hit
GTCATGGGGTCGGTGAGCATGACCCAGGACTAACACACGGGTGGGTCCTG13320.1933441424127665No Hit
GGTAGTATGTAGGCCATTTCCCAGTAGTGTGACCCCACTTTCCATAGCTG13270.1926183761124183No Hit
CAGTAACGTTGGGCAAGTTGTTCTGTCCTCTTGCCACAGCTTCCATGTGG13130.19058623047144327No Hit
TCACTATTCCCATGTCATGGGGTCGGTGAGCATGACCCAGGACTAACACA12350.179264276186011No Hit
GAAAAGAGGAGAGAGGAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCAC12340.17911912292594134No Hit
GGAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCACTCTGGGAAAGATCC11850.17200661318252877No Hit
TTTCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCA11700.16982931428148412No Hit
ATTCCCATGTCATGGGGTCGGTGAGCATGACCCAGGACTAACACACGGGT11440.16605532951967336No Hit
GAGTCAGCCCGTGAGGTGGGGGGATTGCACTCTGGGAAAGATCCATAGAA11430.1659101762596037No Hit
GGCTTAACCACAGGGAGACACAGAGATCTGTCATGAAGATTCCTGGCTTG11310.16416833713876797No Hit
GTGAAGGAGCGGAACAAAAATTGACACGGGGACAGGAGGCAGCTGAGTCA10910.1583622067359822No Hit
ATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCAGTAACGTTGGGCA10420.1512496969925696No Hit
ATGTAGGCCATTTCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAAT9330.13542799164497835No Hit
GTATTTGTTCAGATTCATTCAATTGAAAATGATGGAAACCTGAGTCAACA8990.13049278080261043No Hit
GCTTCCATGTGGGTCAGTGAAGGAGCGGAACAAAAATTGACACGGGGACA8960.1300573210224015No Hit
GAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCACTCTGGGAAAGATCCA8690.1261381830005211No Hit
CTATTCCCATGTCATGGGGTCGGTGAGCATGACCCAGGACTAACACACGG8250.11975143955745675No Hit
CTGGGAAAGATCCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCA8130.11800960043662101No Hit
CTCCTGGAATCCTCCTCCTGTGAGGCCTCGTGTGGTTTGGGGCTGGCGCC8030.11655806783592455No Hit
GAGGGAAGGTGGAAGAGGGAAAGGGGAGAGAAAAGAGGAGAGAGGAATGG7910.11481622871508883No Hit
ATCCAGTTCCATTTGTGCCCCCAGCTCATTCAGTAACGTTGGGCAAGTTG7910.11481622871508883No Hit
GTTCTGTCCTCTTGCCACAGCTTCCATGTGGGTCAGTGAAGGAGCGGAAC7910.11481622871508883No Hit
GAGGAATGGAGTCAGCCCGTGAGGTGGGGGGATTGCACTCTGGGAAAGAT7690.11162285699355666No Hit
GTGGAAGAGGGAAAGGGGAGAGAAAAGAGGAGAGAGGAATGGAGTCAGCC7620.11060678417306913No Hit
GGGAAAGATCCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCAGT7580.11002617113279056No Hit
GTATTCATCACTATTCCCATGTCATGGGGTCGGTGAGCATGACCCAGGAC7510.10901009831230303No Hit
GGATTGCACTCTGGGAAAGATCCATAGAATCCAGTTCCATTTGTGCCCCC7320.1062521863709798No Hit
ATTCATCACTATTCCCATGTCATGGGGTCGGTGAGCATGACCCAGGACTA7280.10567157333070124No Hit
GTGTGTATGTGTATGTGTGTGTACGCACGCCATTTCTTCTTTATCTCCTC7210.1046555005102137No Hit
AAAGCAGGCTGGCCCTGGCTTAACCACAGGGAGACACAGAGATCTGTCAT7050.1023330483490994No Hit
GGACTAACACACGGGTGGGTCCTGCGATCAGGGCCCTGCGCATGGCAGGT7020.10189758856889047No Hit
GGGGAGAGAAAAGAGGAGAGAGGAATGGAGTCAGCCCGTGAGGTGGGGGG7010.1017524353088208No Hit
ATTCTAGTGTGGACCTGTATTTGTTCAGATTCATTCAATTGAAAATGATG6940.1007363624883333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2800.0100.9964451
TAGCCGA300.001935512572.499759
CCAACCG451.1931877E-464.44894
CTGCGCG350.003566082362.142635145
GTCTTAC400.006046754.3827061
GCGAAAG5200.054.3787572
ACCGGAC553.218353E-452.727097
CATATAA1405.456968E-1251.793051
GAGAGTA901.3223416E-648.3366742
ATGAGCG752.5498033E-548.33316145
TAGCACG803.733513E-545.31234145
CGAAAGC6550.044.277873
GAGCGAA700.001055675941.434441
TAAGTAG700.00105605341.4314355
TCGTTGC907.480168E-540.277634145
ATTGCTA1105.2620417E-639.545328
GCAGCGG2400.039.2706958
TAGCTAG1157.1325376E-637.8314441
TCGTGTC1157.139648E-637.8259549
GTCCTAT1354.962876E-737.597921
Produced by FastQC (version 0.11.5)