FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1973-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1973-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844797
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10370.12275138287659638No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10170.12038395022709598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGG1152.4829205E-950.4344948
GTGATCG752.5503303E-548.3330579
AGCGAAA3050.045.1743741
CTCCGAC700.001056577541.428333145
GTTACGT1252.71335E-740.6093831
TATACTG4150.036.690885
GCGAAAG4250.035.8275642
CGAAAGC4900.032.5546883
GAGCGAA950.00472154530.533371
TCGCCAT1504.39808E-528.999834145
GTTATGT6350.028.5499041
GCTACTA1804.699037E-628.1942849
ATGTACG1050.007708000527.6188879
TTACGTG1658.4106025E-526.3666082
GTAATAT3657.2759576E-1225.8278851
TAGCATG11350.025.553542
GTTGACA7200.025.179431
TTGACAT7400.024.4960022
GTAGCAT12850.024.2663081
ATTATAC1801.5188512E-424.1693923
Produced by FastQC (version 0.11.5)