FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1973-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1973-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142996
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1755.2750693E-1141.4283459
GTTACGT1651.3624231E-939.5590821
GTATAAG2404.3655746E-1133.240621
ATAAGAC3102.7284841E-1128.0668223
TATACTG5550.027.4346855
GTTGACA8150.024.9165611
CTACTGA13200.024.168656
GTATATA5150.023.9402891
GTTATGT8500.023.8905851
TACTGAA13300.023.4397227
AGCGAAA4700.023.1462731
TCGCCAT1902.1935164E-422.894613145
GCGAAAG5400.022.8259492
TAGTACT2603.2025455E-622.3095234
CCGACTA3304.6873538E-821.9714984
CAATTCG1650.00260116821.969578145
TTAGAGT5750.021.4365434
ACGAACC4501.3278623E-1020.9461635
ACATGGT9400.020.8261785
TACTGTA3208.466195E-720.3922982
Produced by FastQC (version 0.11.5)