FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1973-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1973-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561566
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT102451.824362586053999No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG75001.3355509414743771No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG68111.2128583283175975No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT63031.1223970112150663No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT52210.9297215287250296No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC48280.8597386593917723No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA39630.7057051174750608No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA38450.6846924493291973No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA37660.6706246460790005No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG33540.5972583810273414No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG30120.5363572580961098No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA25790.45925145040832244No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG24120.42951318277815964No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG23750.4229244648002194No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA22990.4093908819266124No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA21430.38161142234394535No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC20600.3668313252582956No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC20040.35685921156195355No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG19040.3390518656756285No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA18510.32961397235587625No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG17740.315902316023406No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG17690.3150119487290897No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC17290.30788901037455974No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA16250.2893693706527817No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT15810.2815341384627987No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT15440.27494542048485837No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC14700.2617679845289779No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT13730.24449485901924264No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC13260.23612540645266983No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG13000.23149549652222534No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT12970.23096127614563558No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTC12640.22508485200314834No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT12350.21992072169611407No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA12110.21564695868339606No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG12080.2151127383068063No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA12030.21422237101249006No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG11940.21261970988272083No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA11760.20941438762318235No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT11730.20888016724659259No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG11600.20656521228137031No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT11350.20211337580978905No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC11190.19926420046797708No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA11130.19819575971479755No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT11120.1980176862559343No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA10930.19463429053753256No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA10920.19445621707866928No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG10130.1803884138284725No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA9910.176470797733481No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT9570.17041630013213052No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT9420.16774519824918174No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA9190.16364950869532702No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA9160.16311528831873726No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT9100.16204684756555773No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT9060.16133455373010475No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT9030.160800333353515No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG8820.15706079071738674No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG8810.15688271725852349No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA8700.15492390921102772No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA8630.15367739499898497No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT8550.152252807328079No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT8540.15207473386921572No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA8420.14993785236285673No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA8420.14993785236285673No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG8390.14940363198626697No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATC8190.14584216280900197No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT7900.1406780325019677No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC7720.13747271024242919No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG7400.13177435955880518No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC7370.13124013918221544No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG7360.1310620657233522No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT6980.12429527428654868No Hit
CAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCT6920.12322683353336919No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT6840.12180224586246317No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT6790.12091187856814692No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG6750.12019958473269393No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA6730.11984343781496742No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTG6450.11485738096679642No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT6220.11076169141294168No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATA6210.11058361795407842No Hit
GGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCG6030.10737829569453991No Hit
GCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA5950.1059537080236339No Hit
ATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGA5930.1055975611059074No Hit
GAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAAT5910.1052414141881809No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG5850.10417297343500141No Hit
ATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGC5840.10399489997613814No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT5750.1023922388463689No Hit
GAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAA5740.10221416538750566No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG5730.1020360919286424No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC5730.1020360919286424No Hit
ATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATA5700.10150187155205266No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG5670.1009676511754629No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC5660.10078957771659965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA10550.0104.454852
CAGGTAC10900.0101.100793
GGTACTG11000.0100.18175
TACTGAT11900.093.823427
AGCGAAA2550.090.996491
GTACTGA12150.090.699486
AGCAGGT12800.086.108981
ACTGATC13000.085.884518
AGTAGAA15800.083.527431
CTGATCC13250.083.16979
GTAGAAA16900.078.076832
TAGAAAC18150.073.099083
GCGAAAG3200.072.499912
TTTCGTT1000.072.49991145
AGGTACT16650.069.2341464
ACAAGGC13000.066.922998
CGAAAGC3500.066.285633
TTGGACA17500.062.9713484
GCTTTAA15250.062.76521
GACAGTA18050.060.650897
Produced by FastQC (version 0.11.5)