FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1972_lungUR_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1972_lungUR_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047346
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5950.064.591971
GCGAAAG6550.058.6667482
CGAAAGC7700.050.8464363
GCTATAT3450.048.3424221
ATAACCG450.00963937448.33319145
CTATATA4250.047.7668462
TATATAC5550.040.497313
GCCTATA1553.464811E-837.426391
GTATAAG1153.1587158E-431.5276661
CCTATAT2301.0004442E-931.5276661
GTAAGAC5100.029.8542793
TATACGG2054.0378836E-728.293955
TATACTG3850.028.2480185
TCGATGA1306.483268E-427.884533145
ATATACC5400.026.8530544
AAGCCGG1907.1533705E-626.7117215
GTCTTAC4900.026.6376611
CTAGTAC1409.992296E-425.8940163
CGGACCG1409.99508E-425.8927829
CTATACA3657.2759576E-1225.8230744
Produced by FastQC (version 0.11.5)