FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1972-lungUR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1972-lungUR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880249
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG10110.11485386521313855No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG9190.10440227708296175No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC9170.1041750686453492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT608.276402E-1084.597651
TTACGTG1004.7024514E-850.7499472
ATATATC2401.8189894E-1236.2499583
GCGTTAA900.003628017832.2221839
GTATAAG1601.8743012E-631.7241211
CTATATA2202.2599124E-829.6641121
GTATTAT7500.029.0049081
TGCGTTA1000.006074100328.999978
TATATCT4400.028.011334
GTTATGT3650.027.8129251
TAAGGTA2354.296453E-827.7659244
ATTACCT7450.027.2482914
GCATAAT1606.819359E-527.1921021
TATTATC2705.5624696E-926.851822
CATTACC7600.026.7104933
ATATACC1907.154227E-626.7104934
GATTGAC1100.00967102326.3636047
TATTGGG3607.2759576E-1226.1805252
CTCCGAC1409.993705E-425.8928289
GACCGCA1409.993705E-425.8928287
Produced by FastQC (version 0.11.5)