FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1972-lungUL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1972-lungUL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539701
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG6850.12692212910481915No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG5970.11061680448989349No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG5490.1017229910635704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA450.009636848.33327145
TATACTA907.4779135E-540.277735
CCGACTA750.001482789838.666624
GTAGGCT2109.094947E-1237.976146
GTTATGT2451.8189894E-1235.5167431
TCTTTCG900.003626785732.222183145
GTATTAT5000.030.4556031
TAGGCTC2651.382432E-1030.0943017
TATACTG1453.487479E-529.9999625
GTGTACT1952.603574E-729.7435514
CATTCTA5450.029.2660162
ACTACAC1255.1507755E-428.9999623
AGTGTAC1255.1507755E-428.9999623
CTACTGA6100.028.5245536
TACTGAA6350.027.4015397
TTGATTA2705.5515557E-926.851818145
CTACTGT2756.7375368E-926.3684881
GTAGTAT1658.401E-526.3684881
CCAACGT1409.97789E-425.8976231
GTAACGG1409.988686E-425.8928263
Produced by FastQC (version 0.11.5)