FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1972-lungMR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1972-lungMR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586430
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG9040.15415309585116724No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC8350.14238698565898741No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG7410.12635779206384393No Hit
CCCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTA7200.12277680200535443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTA350.003565851162.1424949
AGATCGT400.006047743454.3793146
TATAAGA951.0278901E-438.1609272
GTATATA2002.2191671E-1036.2652471
CGGTTGA850.002739997834.1203584
CTATATC1301.6527209E-533.464193
ACAACGT1153.160625E-431.5215538
GTATAAG1000.006059970229.01221
GTACAAT1000.0060700829.0023023
ACATGGT4500.029.0023025
TATTGGG1358.0749596E-426.8539852
ATATACC1358.0749596E-426.8539854
TAGTGAT1907.1491577E-626.710377
GTTGACA4900.026.6438561
GTGTACT1100.009664644526.365734
GTATTAT3357.4578566E-1125.9810731
TGTATAG1409.98502E-425.8949132
TAGTACC1409.98999E-425.8927069
CATTACC6450.025.8547653
GTAGCAT7900.025.707011
Produced by FastQC (version 0.11.5)