FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1972-lungLR-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1972-lungLR-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981546
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG13640.1389644499595536No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT11400.11614330861722223No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG10600.107992900994961No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG10600.107992900994961No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC10390.10585341899411745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1850.078.4060061
GTCTACG300.001933027272.525561
TTACGTG2900.050.002342
ACGTGTT3600.040.2796634
GTTGACA9150.039.6314471
GATATAC1105.2625146E-639.5473023
CGTGTTG3800.038.159685
TATACTG3800.034.343715
ACATGGT10000.034.076595
TACGTGT4300.033.7225043
CCGACTA3200.031.7202324
GCTACTA1601.8771316E-631.7186159
AACTTCG1204.0580932E-430.2082067
TACGGAT1453.4892164E-530.0014043
GTTATGT5650.029.5236781
GAGCGGT1255.1523634E-429.0013526
AGACGGT1753.7746468E-628.999878145
GTACGGA1804.6981368E-628.1957632
GTATATC1306.4695143E-427.8944451
AGTCGAA1050.00770662627.6203372
Produced by FastQC (version 0.11.5)