FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1972-lungLL-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1972-lungLL-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997050
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG10180.10210119853568025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGT400.006051022654.3746768
CTATATA2350.046.2809682
ACCGTAC1105.2518208E-639.559111
AGCGAAA3450.037.839151
GCTATAT2401.8189894E-1236.2625161
GCGAAAG3750.034.8032882
CGAAAGC4800.028.7006263
TATATAC3301.8189894E-1228.5633053
CCGTACA1606.823731E-527.1900672
ATATACC3204.1836756E-1127.1900674
GTATAAC1409.974457E-425.9017981
GTATACT1801.5193074E-424.168954
GTATATA2451.9063391E-623.6816441
GTATTAT4600.023.6494671
CCTATAT1902.1880407E-422.9026411
TATACTG4409.822543E-1121.4224785
CATTATA2155.052903E-420.2344682
CCTTACG1850.005044110619.5964452
GTAGTAT5707.2759576E-1219.0855331
GTGCTAA11850.018.9727941
Produced by FastQC (version 0.11.5)