FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1971-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1971-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681358
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC8580.125924990973908No Hit
TCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACT6830.10024098931839062No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC6820.1000942235946448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCG259.382833E-487.006125
GTACGAG400.006048377654.3788263
CAATCCG604.944038E-448.3331879
CCTTACG951.9190466E-645.7926942
GTGATCG1251.2645234E-534.7998929
GTATTAT5100.034.120051
GCGAAAG3000.031.4188771
GCTACTA1952.6053021E-729.7435029
CAGTCCG1000.00607320828.9999129
ACGAACC1555.495252E-528.066494
TATACTG3057.0940587E-1026.1493825
GTTGACA4001.8189894E-1225.3767851
TATACTA1751.254552E-424.858892
CGAAAGC3801.2732926E-1124.8043752
GTAATAT2751.9653635E-723.7289411
GCACCGT2451.909424E-623.6751336
TATAATA4002.7284841E-1123.5641572
CGAACCC1851.828961E-423.5151675
CACGAAC1851.828961E-423.5151673
AGCGAAA2802.3387474E-723.305211
Produced by FastQC (version 0.11.5)