FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1970-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1970-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444161
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC6290.1416153151672479No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG300.001935072872.499619
AGCGAAA858.899988E-751.1877171
GCGAAAG1002.7286806E-643.504662
GGTTATA1752.417437E-937.2939071
GTTATAT1406.5930726E-736.2538832
ACAGCGA800.002035453236.24988
ACGAACC1102.4324497E-432.9580764
AAAAGCG900.003624174732.2256745
GTATTGA1306.468914E-427.8907431
GTATTAT3900.027.8907431
TATAACC1855.8017595E-627.4353713
CGAAAGC1606.814761E-527.1904133
CTATTAC1100.0096555626.369431
TATACTG2301.0997792E-625.2200935
TATTATA1500.001492036724.1692562
TATTACC1500.001492036724.1692562
ATAATAC2701.6428748E-724.1692543
TAGGACT2603.1945474E-622.3100834
CGAACCC1650.002596988621.9720525
ATACTGT3304.6698915E-821.9720526
Produced by FastQC (version 0.11.5)