FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1970-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1970-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604423
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA13060.21607384232565607No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10190.16859054006879287No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA8310.13748649538485466No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT7580.12540886101289989No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC7520.12441617873575293No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC7200.1191218732576358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2650.038.308071
GTATTAT2850.038.1640551
GTAGAAA5800.037.5029532
GTTCGTT800.002036018536.249855145
GCGAAAG2950.034.4094922
AAGGTCG1905.4151315E-934.344815
GTCGTTT2051.1370503E-831.829148
TATAACC2301.0004442E-931.5242213
AGGTCGT2106.37192E-931.0738746
GGTCGTT2101.4379111E-831.0713027
GTACCAT1952.6007365E-729.7483921
CGAAAGC3450.029.4226073
TTTCGTT1753.7714835E-628.999882145
TATACTG6300.028.7721085
GTATAAC1050.00770311827.6212223
TAGAAAC8500.027.2962683
GGTTAAC1358.071373E-426.856191
TTAGCGT1358.075275E-426.8539683
TTTAAAC6250.026.68213
AAATATA4100.026.5264785
Produced by FastQC (version 0.11.5)