FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1969_lungMR_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1969_lungMR_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAATTATCCAACATTTATAAATATTTACTGCCCCAGCATGGAGCAAC21.2048192771084338No Hit
GGGTGGCAGTGATTTGGTAGCAGCGCTATCAAATGCTTTTTCTGCATCTG10.6024096385542169No Hit
GATAGTAAGTGTGGCAGGTGATGGTCATGATGAAGATGATGATGGTTTTC10.6024096385542169No Hit
GTCCCTGTCATCCCCCACGCATCCATTACCTCTACCTGCAGAACAACTTC10.6024096385542169No Hit
AGCGAAACCAGGCAGAAGAAGAAGTAGGCTCCCTCCTGAGCAAGTGGGAC10.6024096385542169No Hit
CCCAGGCGCCCACTAATGAAAATCGATTTAAACTTTCATAAAAGATGGAC10.6024096385542169No Hit
GTTAAAGCCAAGGAGTGCCAAGGATTCCTTGCATTCCAAAGACATTCCAG10.6024096385542169No Hit
GGTTAATTTCCACATATTCGTGAATTTTCCAGTTTTCCTTCTGTTACTGA10.6024096385542169No Hit
GTGGCAGGCAGAAGCAGAGAGAGAAGCAGGCTCCCTGCTGAGCAAGGAGT10.6024096385542169No Hit
GTCTTGTGTGTTGGCCCAAGTAAACTCTTGGGAGGGTTGTTAATTGCAGG10.6024096385542169No Hit
GAATATTACTCAACCATCAGAAAAGATGAATACTTACGGTTTACACATAA10.6024096385542169No Hit
GGTTCAAGCACTCTCAAGGTAGAAGTCATGCTGCTGCCCAGAGCAGTGGT10.6024096385542169No Hit
TGAGTGAACTGAACAAGTCATAAATAAGGCGCTCCCTTCTTGTCTACAAT10.6024096385542169No Hit
CACCGTAGCCAACCCGCTCAGGCCTCGGGGCTGGCAACCTCTCTAGGCAG10.6024096385542169No Hit
CCCCCCGGGGTGGCTGACCAAGCCCCCAGGGCTGGCACAGGACAGCCCTC10.6024096385542169No Hit
CTATAGAAGATCGAATAATAAAACCAAGACTTTCCGTACAATTTTGTGTC10.6024096385542169No Hit
GGGGGGGGGGGGTTAAAAAATAAAAAGGTGGGCGTGGGGCGTGCTTACCC10.6024096385542169No Hit
TTGTGAGATTGAGGCTTTGTCATCTTGTGTGTGTGTGTGTGCATGTGTGT10.6024096385542169No Hit
GCACGGGATGGTTCAAAAGTTAGGTTTGGCCCCCGGTGAGCGGGAAGCCA10.6024096385542169No Hit
GAACAGAAACCATCTGATGTGCACTATTATCTCTCCCATACTTCACCTTC10.6024096385542169No Hit
CCATAGGGAGCCATAAAGGTCTTCATGTCCCACTGGCAGGAGACCAAATT10.6024096385542169No Hit
TCACAGACCTGTACCCCTGGGGCTAATAATACATTATATGTTTATTTAAA10.6024096385542169No Hit
GGGGCGGGTGTAAACAATGAAAGGAAGCAGGAGCCTGGAGAATCTTGCGT10.6024096385542169No Hit
ACAGCCATCAGACTGGCTGCCAAATCAGCTCTCAGAGGAATTGTATACAA10.6024096385542169No Hit
CCTCACGTTGCTGTGAATGTTAAATAAGTGTTTCTATTGAACAGATCCAG10.6024096385542169No Hit
TGGTCCAGGTGACACACCTGCAGGGAGGAGACACAGGAAGTTACTAGCCT10.6024096385542169No Hit
GGGAAAAGCAGAGGCAAAGCAGCAGAGTTGCAGGGCGGTGCAGGGGGGTT10.6024096385542169No Hit
GTCCTGGGATCGAGCCCCAGGTCAGGTTCACTGATCGGCATAGCACCTGC10.6024096385542169No Hit
CCCCTCACCCGTCCCCGGCTCCTGACATCCTTGCTTCCAGCCAGGTCGCT10.6024096385542169No Hit
TCTCAGAGAAGCCCTACCCTCCCTCTCTCTATTAAACCGCTCTGTTTTGT10.6024096385542169No Hit
CCTCCTGAGCAAGTGGGACTTGATCCCAGGACCCACAAAATGTGACCTGA10.6024096385542169No Hit
GGTGCAAGCCCTTGAAGGGGAATGAGAGAGACATGCTCTGGGAGCTCAAC10.6024096385542169No Hit
ATATTTAGGAGGACTACGTGGCAACAGGTACGGATTTAGTGTTTCTTGAC10.6024096385542169No Hit
GTGGGAAACCTGCTTCCCCCTCTCTCTCTGCATGCCTCTCTGCCTACTTG10.6024096385542169No Hit
GGGGAGGCTGGAGGGAAGAAGGCAGGGAGCGGGAGTGGTAGGTGAGGACT10.6024096385542169No Hit
CCCCAGGGCTTCCTCGTGGACTAGTTCGCGCACGTGTGCATACCTGCTGG10.6024096385542169No Hit
GCACAGGGTGTGGGGACCAGCCAGGCGGCAGAAGCACAGGTCGGGGTGGC10.6024096385542169No Hit
TGACAAGTCTTACTGTTTCGGGTAAATGTGGATTTAAATTTCCTTGAACA10.6024096385542169No Hit
GGGGTGGGTGGGAAGAAGGGAAGGTGGTAGAGATGGCCAGGGTTTTATTT10.6024096385542169No Hit
GGGATTAATGCAGTAAAATGATGCAACATTTATAAATATTTACCCCCCCA10.6024096385542169No Hit
CCCTAACTACTTCTGAAATTAGGCCTCCTGTTTCCATTTTTGGAGCTAGC10.6024096385542169No Hit
CTCTTCCATGGTTTTTCTAGGCACTGAGAAAAATCTCCCAAGTTAAGCAT10.6024096385542169No Hit
CTGCTGTCCTGCCCGGTCCCTGCAAGCTCGCTGCCCATTTCGTAGTCTTT10.6024096385542169No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG10.6024096385542169No Hit
CATATAGACCTATGTTCAAAACCAAACACCACCATTTATGAGCTGTGTGC10.6024096385542169No Hit
ACTCTGAGCCCCGGACGTGCGCAAAACTTCTAGATGGAGCTGGAGTCCAC10.6024096385542169No Hit
GCTGCGGTCTGGCGCCCTCTGCAGGCGGTGGAAGGCAGTGCAGGACTGTA10.6024096385542169No Hit
GGTTAAGAGGGAGAGGGAGAAGCAGACTCTACACTGAGCATGAAGCACAA10.6024096385542169No Hit
CATTTGAATATCATCAAACTTCTCTCTATTATACAGAACCCAAATTCAGG10.6024096385542169No Hit
ATACACATCCTCGTATTGGAGTCAGGATATGGTAATTGGAGAAGAATTTA10.6024096385542169No Hit
GGGCCAGGGTTTTCATTTGGAGTCACACAAGAATCACAGGATATCAGAAT10.6024096385542169No Hit
GTCCTGCCCTATGGCCCGGGCTGCTGGAGTGCCCGTGCTCTGCCTTCACC10.6024096385542169No Hit
GGTTGAACAAGGCAATGCCATGCCTTCTTGCTTCAGCCAGCTCTCACATG10.6024096385542169No Hit
GCCCGGAACTCGCAGAAACCCCTACTGGTTGAACGGGCAATCAGCTGCCG10.6024096385542169No Hit
CTGTTGTAGTTCAGGCGAATGAAGGCAAGGTTGGGGAAACCCTTGAAGTA10.6024096385542169No Hit
GAACAACTTCATAACGGAGCTCCCCGTGGAGTCCTTCCAGAATGCCACCG10.6024096385542169No Hit
GTCCAGACCCACAGACCAGATGTCCTAACCAGGCCATAGGCCAAATGGGG10.6024096385542169No Hit
CCACTAAACAATAACTCCCCATTCCTCTCCATACCCCCAGCCCCTTCAAA10.6024096385542169No Hit
CCCCAGCAGAGCGCCGGAGAGCCCAACTTGGGGCTCACGCCCAGGCTCGA10.6024096385542169No Hit
GTTGACTCTCTGTCTCACTCGTCACTTTTCATCAAATCCAACTGGCAACT10.6024096385542169No Hit
GTAGTCAATTAGGGGCCCTAGCCATTCCTTTTTTTTTTTTTAAATATTTT10.6024096385542169No Hit
CCTCGGGATATCATGGGTAGACACATAACACACCAGCAAGTACAACGACA10.6024096385542169No Hit
GTCTGACAGCCTGTTGTAGTTCAGGCGAATGAAGGCAAGGTTGGGGAACC10.6024096385542169No Hit
GTCCTCTGATCATGGTTATTTATTTTTTATTGTACTTACATGACACAAGG10.6024096385542169No Hit
TCAATGAGCCATGTGTGCTCTAAACACGTCTTCACAAAATCCGGGCCATG10.6024096385542169No Hit
GTATTTAAACTCTGGACGTGTCTTCTTGCAGTGACCGCGGCGGATGGGGG10.6024096385542169No Hit
GTTTCAAGGCTTACCTCCTCTGGATCTTGAGCAACCAACACTGTAGATGG10.6024096385542169No Hit
GGGATACTGAGCTCACCTCCACTCCGTGATGTTGTGCACCTCGTGTGTAT10.6024096385542169No Hit
GTCATGTCCACCAGGGACGCAGAGCTGGAGGAGTTCCTGCCACTCACGAC10.6024096385542169No Hit
GTTTCAAGATATGTGGAAATTGTGTGAAATGAAAATTTCAGCACAAGTTA10.6024096385542169No Hit
ACTAAGCAGAGAGCCTGATGCGGGGCTCGATCCCAGGACCCTGCCATCAT10.6024096385542169No Hit
CTGGGACTTGCTGGAGAAGACCCTTAGCCTCTCAAGTGCTTGGTCTCCCC10.6024096385542169No Hit
CCAGCTAGATGAGTTTGCTGGGGCTGCCATAATGGACTGCCCACAGGCTG10.6024096385542169No Hit
ACCTTATTGTTGACTCATTAACATTGACCTCACGCACAACACTATAACTT10.6024096385542169No Hit
ATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCT10.6024096385542169No Hit
GTCATCCCCCCACGCATCCATTACCTCTACCTGCAGAACAACTTCATAAC10.6024096385542169No Hit
CAGCTAGAGAGTTAAGATCCTGTGGGATCAGATGTGCCTACCTCTCCCAC10.6024096385542169No Hit
CGCTTCACCAACTGAGCCACCCAGGCACCCTAAAATATTTTAATTTCTTT10.6024096385542169No Hit
GTCCACAACCATTGGTCCTTTTCTCCATCTGTGTTCAGAAGAGACTTACT10.6024096385542169No Hit
CCCTTGAAGGATTACAATGACTATGGCTGCTACTGTGGCCTGGGTGGATC10.6024096385542169No Hit
CCTTTGTTTTGTGAATGCGGCACATTAGTTTGATTTGTGGATGTTGAACC10.6024096385542169No Hit
GGGGGCAGCTGGAGATGAGAAATTCAGGGCCGCGGTGTGCGGGGCTACCG10.6024096385542169No Hit
GGGTGGGGTTCTGGGTGTGGCACGACGACTGGGTTAAGAGGCAACTCTGG10.6024096385542169No Hit
GCTAACAGTGAGGCTGGGAATCTTGGTTATGAAGTTACGAAGCCAGGAAC10.6024096385542169No Hit
CATCAGGCTCCCTGCTCAGCAGGGAACCTGCTTCTGCTTCTCCCACGCCC10.6024096385542169No Hit
AAGTCCAGGACCCCATGGTTTCACCTGTGAATTCTATGAATCATCCACAG10.6024096385542169No Hit
ACATATGACCTAAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATA10.6024096385542169No Hit
GGTTTCATCTGGGCCAATCATGGGGAATGTTTCTTTGTGAATAAAATCAA10.6024096385542169No Hit
GTCTGACAGCCTGTTGTAGTTCAGGCGAATGAAGGCAAGGTTGGGGAAAC10.6024096385542169No Hit
CTGCGGACACAGCTCCGCCGCTGAGGGGAGGGGCTGCCTCAGGGTAGTTC10.6024096385542169No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA10.6024096385542169No Hit
TTACTAGCCTGCTGGACCTCTGGAGTTAGAGGCCCTGTCTTGGCACAAGA10.6024096385542169No Hit
GGCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTA10.6024096385542169No Hit
GTCCAGGGAAGTTAGGGCACTGAGAGGAGAAGGTCGCGATCCCATGGCCA10.6024096385542169No Hit
GAGAAGGATTGGTATTCATTTTTTTTTCTGTGGATGATTCGTAGAATTCA10.6024096385542169No Hit
GTCTAATATGGGGCTCGGACTCCTGACCCGGAGAACAAGCGTTGCACGCT10.6024096385542169No Hit
GGATTGGGCAGGGGATCGTTCTCCACTCTGAGGAAGGAGTCTGGGCAGAA10.6024096385542169No Hit
GTGCCGGGGCAGGGCCGAGGGCACCTCTTCGAGCTGGTTCTTCTCCATGT10.6024096385542169No Hit
GTGAGGATGCTGGACGATAGCCGGGACCCCACACCGCTGCTCAAGGAGAT10.6024096385542169No Hit
GTCCCACACCTGCCCCCTTCCTGGGGAGCAAGCCCAGCCCAACTCGGCCA10.6024096385542169No Hit
GGTCCTACCTGGGCCACGAGAAACACCGCCGCAAGCTGAGCCACTCAGAC10.6024096385542169No Hit
GTGCAGGGGGGTTGCAGAGGGCCAGACGTGTGAGGGGCTGCGGTGGTAGG10.6024096385542169No Hit
CCCCTCTATCTTCCCTGACTGCCCCCGGGAATGCTACTGCCCCCCGGATT10.6024096385542169No Hit
GATTTTAATCTACCGTATGATGGGAAGCCACAGAGACAGGATGACAGGGT10.6024096385542169No Hit
GGCTGCTCTTCCCCCTGGGCCATTTCTCAAGGTTGTGTTTTCAGCAAGCT10.6024096385542169No Hit
TCACTACCCAACACATTATTCCAAAAGGAGATTCCAGAAGTCAAGACTCC10.6024096385542169No Hit
TACATGCTTTGGCCAAACCTCTCTACACAGCAGATTTAAAAATACAGAGT10.6024096385542169No Hit
GTGCTAATGGCGATTCAGAATTATGTAAATCACTAAAAAAAATTAACATA10.6024096385542169No Hit
GAACACAAGCAGGGGGAGAGACACAGGGAGAGGGAGAAGAAGACTCCCTG10.6024096385542169No Hit
GTGTCGAAGAGCACGGGCCCTGTAGACCCTGGAGCCAGACAGACCTGGAT10.6024096385542169No Hit
GCTTAAACAATAAGCCACTGATGAATCATCTATTAAGGACTTTGGCACTA10.6024096385542169No Hit
CCGCGGGCCAGTGGCAAGCGTCACCCGCCTCCTGTGCCAAGCGACGGAAG10.6024096385542169No Hit
GCTTCAGGGCGGGCTTCCTGGAGGAGGGGGTATCTCAGCGGAGACCAGAA10.6024096385542169No Hit
TTACAAAATACACCGGTAGGTCATATTTGGCCTGGGGCCCTCAACCTTAA10.6024096385542169No Hit
GGCTTGAGATGGGGGGTGTGGAGAGAATGCCTAGTGGGTTGCTTGTTCTC10.6024096385542169No Hit
CGAAAGCAGGGGGAGCAGCGGAGAGAGGGAGAAGCAGACTCCCTGATGAG10.6024096385542169No Hit
GAATTATGTTCATATTTTAACTCAACAATGCTGGCAAGTTTGTATTATCT10.6024096385542169No Hit
TCTCAGTGCCGATCCCGTGGAGCCGCGGCAAAGAGACCTCTGGCCTCCCC10.6024096385542169No Hit
TTGTAATCTAGGTTTTCTCCATCCTCCTCAATCTTCTCCTACTGGTTCCA10.6024096385542169No Hit
GGTAGAGCTCAATAAGAGTGAGGAGTAGCTGAGTGCCCCAGACCCCAAAG10.6024096385542169No Hit
GTAGGTGCTTGATCCTTGGTGCTTTGAGGAGCCTTACAAAAAGCATCTCA10.6024096385542169No Hit
GGCACATGTACACACACAGACACAGACACAGACACAGCCACACGCACACA10.6024096385542169No Hit
GGCTGGGGTATGGGTCCCATGGAGGACACCTGGTAGGAGAGTGAGCTGCT10.6024096385542169No Hit
CTGCCAAGCAGAGAGCTCGATGCAGGGCTCAATCCCAGGACCCCGAGATC10.6024096385542169No Hit
GCGGGAGCAGGTGTATGCCCCCCTCGAGGCCTACACAAAGCAGAAGTATC10.6024096385542169No Hit
TCTGGGAATTGATTTCTAGTTCTATAACAATGTGGTTGGAAAAGATGGTT10.6024096385542169No Hit
GTTTCCCACAGAGCAGGGGGCCCCCCAATGCAGGACTCGATCCCAGGACC10.6024096385542169No Hit
GGCCCGCAGGGGACCCGCGCATCCCTGCCCCTGGCGGGCAGCCCGTCGTC10.6024096385542169No Hit
CACAACACACACACAGCACACGCTACACAGAAACCATATACCACACATAG10.6024096385542169No Hit
TCCCAGGGCCTGCTGGATTTGCTCACGTATGGGTGAGGGAACAGGCAGGG10.6024096385542169No Hit
GTTAGGAAAGCCTCACAGACTTGGCTGACTGCATAAAGGAAGAAACGCAA10.6024096385542169No Hit
GCATTAAGGGTTTCATGTGATTCAGCGAATCATAAGGCAATGCCACCTGT10.6024096385542169No Hit
ACCTTAAGTATCACTTCACCTGGCTGTTCACTGATATTCTTTGTAAGGAT10.6024096385542169No Hit
CCACAGAACTGGGGGCACAGTCAGAAAACAACTGAATTGAGGGATCTCTA10.6024096385542169No Hit
GATCATCGCCAGCCCTGGGGCCCTTTGATATTTATAAGGGTTGACATTTT10.6024096385542169No Hit
CTTTGCTTGGGCTCTGAAGCACTTTCTTTCCCGGGTGACGCAGCTGCAGC10.6024096385542169No Hit
GTGCGGCTCGCGCCAAGCTGAAAACCTCTGTGAAAGCATAGCATTCACTG10.6024096385542169No Hit
CATTAAGGGTCATCTCTCATGTTAACCAGGGAATTGCTTCAGAGTCTTCA10.6024096385542169No Hit
AAAGAATTCAGTCTTGATTGTATTTGTCTTTATTCATCTGCAGTGGTAGG10.6024096385542169No Hit
GAACTGTGTCTTCAGTGAGTCAGGAGTGGCTCCTCAAACTTGCTTAGGCT10.6024096385542169No Hit
CTGGAAGGCTCTGGACCATTCTTGAAAAGAGCAACTGTCAAGCAGCCAGC10.6024096385542169No Hit
GTATAGTACCCTTCCTGGCAATTTGTGTAACTCCCATAGCCTATACTAAT10.6024096385542169No Hit
GGCTATACAGGAAACAGCCTCTGAAAACAGAGATATCATCTCTTTTCAGA10.6024096385542169No Hit
GGCTGAAGCGCAGAGAGGGGAAGAGACCTACTGTCATACATTGTGATCAC10.6024096385542169No Hit
AGGCTGATGTTTCCGTTGTGATCTTCTGTCTGGATGGGGGATCATTGAGA10.6024096385542169No Hit
GCCAGGTAGTATGTAGGCCATTTCCCAGTAGTGTGACCCCACTTTCCATA10.6024096385542169No Hit
ACGCTTGACTGACCGAGCCACCCAGGTGCCCCTTTATGACTTTAAATTAC10.6024096385542169No Hit
CTCCTGAGCTTCCTCACCTTTTTACTCCTTGATACTCTGAAATCTCAAGT10.6024096385542169No Hit
GCAGGGGGAGAGGCAGGCAGAGGGAGAGGGAGAAGCAGGCTCTCCACAGA10.6024096385542169No Hit
CGGCTCACCTGCTCAGCTCCTGCGGCCACGTGGCCGCCCAGGGCTGCTTG10.6024096385542169No Hit
GGCCCTGCTCAACGCATTTGAAGTCCCTGGGAAGCCCAGGGCCTCCGGCA10.6024096385542169No Hit
GTCAGTGGAGCTCTGTGACTCCTGATCTTGTGGTTTTGAGTTTCAACCCC10.6024096385542169No Hit
CAGGTAGGATGTGGCCGGGCTAGTCCCCTTCGCTGCAGGGATGGAGGTGG10.6024096385542169No Hit
GGACCGAGCACGGAGGCAAACAGCCGGACGCCAAGAAAAAGAATCCATGG10.6024096385542169No Hit
GTTGCTGTCCAGGTAGAGCTGGTGAATGGCCGTGGGCACCTTGGGGGGCA10.6024096385542169No Hit
GTTGTATCCTGGTGCTTGTTGGCAATGCCCAAGCCGGGCCAAGAGGAGGC10.6024096385542169No Hit
CCCCTACAGTGTCTCCAATGTCTCCGCCTCACATTCCACATTGCTGCACC10.6024096385542169No Hit
GACTTGGCTGACTGCATAAAGGAAGAAACGCAAGTCAGAGTTGTCCCCGT10.6024096385542169No Hit
TGCAAGGAGTGCCCATTATGATCACTGCTCCCAAATCAACTTAATATACA10.6024096385542169No Hit
GGGCAATGGAGGTTGTGGGGGTGGTCAAAACACATGTTGGGTGGGTGGTT10.6024096385542169No Hit
GGGGTGGGAATGCCCTTCCGGTTCAAGTCCTAGTTCAGGAGCAGAGACAC10.6024096385542169No Hit
GAGGAAGGCTGGGTGTGGGCCTGAGTTTGGGTCTGGGCCTGGGCCTGGGT10.6024096385542169No Hit
ATAAGATCATCTGTTAGTTCTGCAGCCATGAACAAAGCACACCCCTGCTC10.6024096385542169No Hit
GGCTGGGTGTGGGCCTGAGTTTGGGTCTGGGCCTGGGCCTGGGTCTTGGT10.6024096385542169No Hit
TCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAAGATGGTT10.6024096385542169No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers