FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1969_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1969_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467561
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC5770.12340635767311645No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG5730.12255085432702897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCTA2057.2759576E-1238.9103855
GTTACGT750.001480311238.6787071
GCTATAT1157.11933E-637.8378641
CAATTCG950.0047249730.526022145
AGCGAAA1702.9999082E-629.8622381
ACGTGTT1000.00606513629.0059264
TATACTG1753.7629688E-629.0059265
TAGTATG2054.0241866E-728.2984642
CGACTAG2104.9680057E-727.6246916
TACGTGT1050.00769664227.6246913
AGTCTAT1358.061568E-426.8602121
GTCTATT1907.13344E-626.7159842
TCAGGCG1907.145307E-626.710279
GTAGTAT2259.0654794E-725.7858051
CTATAGA2001.061321E-525.38291
GCGAAAG2351.3243116E-624.6885361
TATAATA3052.0243533E-823.7753492
TATAGAG1902.1877133E-422.8994142
GTTGACA5100.022.752181
GTTATGT3203.3647666E-822.6633031
Produced by FastQC (version 0.11.5)